MilliporeSigma™ HLA Class I Antigens, Mouse, Unlabeled, Clone: W6/32,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN1783MI-Each-of-1 | In Stock | ₹ 40,633.84 |
MABN1783MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,633.84
GST (18%): ₹ 7,314.091
Total Price: ₹ 47,947.931
Antigen
HLA Class I Antigens
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Gene Accession No.
P30443;Q96DW9;O19617;P13747
Immunogen
Membrane from human tonsil lymphocyte preparations (Barnstable, C.J., et al. (1978). Cell. 14(1):9-20).
Quantity
100 μL
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_001229687;CAD30340;NP_001229971;NP_005507
Target Species
Human
Form
Purified
Applications
Affinity Purification, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Neutralization
Clone
W6/32
Conjugate
Unconjugated
Gene
HLA-A, HLAA, HLA-B, HLAB, HLA-C, HLAC, HLA-E, HLAE
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone W6/32 targets HLA classe I heavy chains (HLA-A, -B, -C, -E, and possibly -F) in a light chain-dependent manner, although clone W6/32 does not exhibit affinity toward isolated light chain (β2-microglobulin or β2-m). A tight interaction between β2-m residue 89 and some residues within the heavy chain second domain might be responsible for the formation of the W6/32 antigenic determinant which, however, is only expressed (or exposed) when β2-m cannot covalently bind to the heavy chain Cys121 (Kahn-Perles, B., et al. (1987). J. Immunol. 138(7).2190-2196). Cell surface MHC I molecules reconstituted with allele-specific peptides and recombinant human β2-m that contains a methionine at the N-terminus is not recognized by W6/32 (Shields, M.J., and Ribaudo, R.K. (1998). Tissue Antigens. 51(5):567-570). Clone W6/32 is reported to bind murine, rat, rabbit and guinea pig HLA class I heavy chains only when they are associated with bovine β2-m, but not with their
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype
IgG2a
Related Products
Description
- Major histocompatibility complexes (MHCs), also called human leukocyte antigens (HLAs) in human, are cell surface molecules that play a major part of the immune system in all vertebrates by determining histocompatibility
- The main function of MHCs is to bind and present peptide fragments derived from pathogens on the surface of antigen presenting cells
- MHCs are divided into three classes, class I MHCs are heterodimers composed of a transmembrane alpha chain and a common extracellular beta subunit
- The alpha chain contains three domains (alpha1, alpha2, and alpha3), with the alpha1 domain mediating interaction with the beta2 subunit
- The alpha1 and alpha2 domains form the antigen-binding groove
- Class II MHCs are heterodimers of two single transmembrane chains, alpha and beta, each contains two domains, with the N-terminal alpha1 and beta1 domain from each chain forming the antigen-binding groove
- Class III molecules include several secreted proteins with immune functions, including components of the complement system, cytokines, and heat shock proteins
- Each human cell expresses six MHC class I alleles (one HLA-A, -B, and -C allele from each parent)
- Classical MHCs present antigens to the TCRs of CD8+ T lymphocytes
- Nonclassical molecules (class IB MHC) exhibit limited polymorphism, expression patterns, and presented antigens
- This group is subdivided into a group encoded within MHC loci (e.g
- HLA-E, -F, -G) and those not (e.g
- ULBPs, Rae1, and H60).