MilliporeSigma™ Mouse anti-Calpain I/II, large subunit, Clone: 1D10A7,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS1215MI-Each-of-1 | In Stock | ₹ 40,292.08 |
MABS1215MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,292.08
GST (18%): ₹ 7,252.574
Total Price: ₹ 47,544.654
Antigen
Calpain I/II, large subunit
Classification
Monoclonal
Formulation
Mouse monoclonal IgG2aκ ascites with 0.05% sodium azide.
Gene Symbols
CAPN1; CANPL1; PIG30
Immunogen
Purified m-calpain from rabbit skeletal muscle.
Quantity
50 μL
Research Discipline
Signaling
Gene ID (Entrez)
NP_001185797
Content And Storage
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Isotype
IgG2a κ
Applications
Immunocytochemistry, Western Blot
Clone
1D10A7
Gene Accession No.
P07384
Host Species
Mouse
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Chicken, Human, Monkey, Rabbit, Rat
Form
Ascites
Related Products
Description
- Specifically detects Calpain I/II, large subunit clone: 1D10A7 in Chicken, Human, Monkey, Rabbit, Rat samples, and it is validated for Immunocytochemistry, Western Blotting Calpains I & II are intracellular cysteine proteinases distributed in various tissues and cells
- Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca 2+ concentrations and calpain II at millimolar Ca 2+ concentrations
- Both calpains are composed of a large catalytic (80kDa) and a small regulatory (30kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus
- Upon Ca 2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis
- Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes
- The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4
- To date, calpain I & II remain the best characterized members of the calpain family
- Calpain activity was initially characterized as 'calcium-activated neutral protease' (CANP) detected in brain, lens of the eye, and other tissues
- The name 'calpain' was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.