CRB3A, Rat anti-Human, Clone: 1E6, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT136625-Each-of-1 | In Stock | ₹ 17,118.26 |
MABT136625 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
CRB3A
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
CRB3;UNQ588;PRO1158
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein crumbs homolog 3 (CRB3).
Quantity
25 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_631900
Target Species
Human
Form
Purified
Applications
Immunohistochemistry (Paraffin), Western Blot
Clone
1E6
Dilution
Western Blotting Analysis: 2μg/mL from a representative lot detected CRB3A in Caco2 cell lysates over-expressing Crumbs 3A (positive) versus lysates from Caco2 cells silenced with siRNA (negative).Western Blotting Analysis: A representative lot detected CRB3A in Western Blotting applications (Loie, E., et. al. (2015). Mol Cell Biol. 35(19):3423-35).
Gene Alias
Protein crumbs homolog 3
Host Species
Rat
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 1E6 is a rat monoclonal antibody that detects protein crumbs homolog 3 in human cells. it targets an epitope with in 15 amino acids from the C-terminal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-CRB3A, clone 1E6, Cat
- No
- MABT1366, is a rat monoclonal antibody that detects Protein crumbs homolog 3 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Protein crumbs homolog 3 (UniProt: Q9BUF7) is encoded by the CRB3 (also known as UNQ588/PRO1158) gene (Gene ID: 92359) in human
- Mammalian cells contain three Crb orthologs
- CRB1 is primarily expressed in the brain, cornea, and retina
- CRB2 is expressed in lung, kidney, retina, colon and mammary glands
- It is also expressed in at moderate levels in Expressed at moderate levels in liver, spleen, pancreas, placenta, and prostate tissue
- CRB3 is present in epithelial tissues and is present in two isoforms, CRB3A and CRB3B
- CRB3 is a single-pass type I membrane protein that is reported to control epithelial cell polarization
- CRB3A is apically localized and promotes apical membrane growth and is and is essential for the formation of tight junctions in epithelial cells
- It also prevents the epithelial-to-mesenchymal transition (EMT), which is known to contribute to tumor progression
- CRB3 is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off
- The mature form contains an extracellular domain (aa 27-59), a transmembrane domain (aa 60-80), and a cytoplasmic tail (aa 81-120), which contains a PDZ-domain binding motif (aa 117-120) that interacts with the Maguk protein, PALS1, which recruits PALS1-associated tight junction (PATJ) protein into the CRB3A complex
- Both PALS1 and PATJ play a critical role in epithelial cell polarity and tight junction formation
- CRB3A is shown to control and properly localize RhoA signaling that is required for CRB3A-dependent reorganization of the actin cytoskeleton
- (Ref.: Loie, E., et al (2015)
- Mol
- Cell Biol
- 35(19); 3423-3435).