Podoplanin, Mouse anti-Monkey, Human, Clone: LpMab-12, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT84125UG-Each-of-1 | In Stock | ₹ 17,118.26 |
MABT84125UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
Podoplanin
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
PDPN;GP36;PSEC0003;PSEC0025
Immunogen
LN229 glioma cells expressing human Podoplanin.
Quantity
25 μg
Research Discipline
Cell Structure
Test Specificity
Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone
LpMab-12
Dilution
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
Gene Alias
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Monkey, Human
Form
Purified
Related Products
Description
- Anti-Podoplanin, clone LpMab-12, Cat
- No
- MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin)
- Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human
- Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes
- It may be involved in cell migration and/or actin cytoskeleton organization
- Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles
- Six isoforms of podoplanin have been described that are produced by alternative splicing
- It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form
- Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162)
- Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus
- Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2)
- Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3
- Clone LpMab-12 specifically detects sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin
- It also reacts with the synthetic glycopeptide of hPDPN, corresponding to 38 54 amino acids that carries 2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52
- The minimal epitope of LpMab-12 is identified as Asp49 Pro53 of hPDPN
- (Ref.: Kato, Y et al
- (2016)
- PLoS ONE 11(3): e0152912).