NBP177701

AKT1, p Ser473 Antibody (17F6.B11), DyLight 488, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP177701-Each-of-1 In Stock ₹ 66,171.50

NBP177701 - Each of 1

₹ 66,171.50

In Stock

Quantity

1

Base Price: ₹ 66,171.50

GST (18%): ₹ 11,910.87

Total Price: ₹ 78,082.37

Antigen

AKT1 (p Ser473)

Classification

Monoclonal

Concentration

LYOPH

Dilution

Western Blot 1:500 - 1:3000, Flow Cytometry 1:10 - 1:1000, ELISA 1:20000, Immunohistochemistry 20 ug/mL, Immunocytochemistry/Immunofluorescence >1:5000, Immunohistochemistry-Paraffin 20 ug/mL, Fluorophore-linked immunosorbent assay 1:100-1:2000

Gene Alias

AKT, AKT serine/threonine kinase 1, AKT1m, CWS6, EC 2.7.11, EC 2.7.11.1, PKB, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinas

Host Species

Mouse

Molecular Weight of Antigen

55.7 kDa

Quantity

0.1 mg

Research Discipline

Apoptosis, Autophagy, Cancer, Growth and Development, Hypoxia, mTOR Pathway, Neuronal Cell Markers, Phospho Specific, Signal Transduction

Gene ID (Entrez)

207

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Content And Storage

Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.

Isotype

IgG1 κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone

17F6.B11

Conjugate

DyLight 488

Gene Accession No.

P31749

Gene Symbols

AKT1

Immunogen

AKT1 [p Ser473] Antibody (17F6.B11) was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT11 protein, followed by hybridoma development. (Uniprot: P31749)

Purification Method

Protein A purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

Target Species

Human, Mouse, Rat, Monkey

Form

Purified

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Description

  • AKT1 Monoclonal specifically detects AKT1 in Human, Mouse, Rat, Monkey samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Fluorophore-linked immunosorbent assay.