Caldesmon/CALD1 Antibody (CALD1/820), Novus Biologicals™

Caldesmon/CALD1

Monoclonal

0.2mg/mL

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 1 - 2 ug/ml

Q05682

CALD1

Recombinant full-length human CALD1 protein

Protein A or G purified

RUO

Primary

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Store at 4C.

IgG1 κ

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CALD1/820

Unconjugated

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

CAD, caldesmon, caldesmon 1, CDMH-CAD, HCAD, LCAD, L-CAD, MGC21352, NAG22

Mouse

150 kDa

0.2 mg

Cytoskeleton Markers

800

Human, Rat (Negative)

Purified