Fas/TNFRSF6/CD95 Antibody (B-R18), Novus Biologicals™

Fas/TNFRSF6/CD95

Monoclonal

0.2 mg/mL

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

P25445

FAS

Recombinant human CD95 protein

0.2 mg

Apoptosis, Cancer, Cell Biology, Death Receptor Signaling Pathway, Diabetes Research, Inhibitors Activators and Regulators

355

Human

Purified

Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence

B-R18

Unconjugated

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Apo-1 antigen, apoptosis antigen 1, Apoptosis-mediating surface antigen FAS, APT1FASTM, CD95 antigen, CD95ALPS1A, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, FAS1, FASLG receptor, TNFRSF6member 6, tumor necrosis factor receptor superfamily member 6

Mouse

Protein A or G purified

RUO

Primary

MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

Store at 4C.

IgG1 κ