MVP Antibody (1032) - Azide and BSA Free, Novus Biologicals™

MVP

Monoclonal

1.0 mg/mL

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

LRPVAULT1, Lung resistance-related protein, major vault protein

Mouse

Protein A or G purified

RUO

Primary

Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IgG1 κ

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

1032

Unconjugated

PBS with No Preservative

MVP

Proteins precipitated from human breast cancer MCF-7 cells

0.1 mg

Cancer

9961

Human

Purified