Bcl-2 Antibody (124), Alexa Fluor™ 750, Novus Biologicals™

Bcl-2

Monoclonal

Alexa Fluor 750

50 mM sodium borate with 0.05% sodium azide

BCL2

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein (Uniprot: P10415 )

0.1 mL

596

Human, Mouse, Rat

IgG1 κ

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

124

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Mouse

Protein A or G purified

Primary

This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Store at 4°C in the dark.