TPH1 Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PA14678-Each-of-1 | In Stock | ₹ 48,905.50 |
PA14678 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,905.50
GST (18%): ₹ 8,802.99
Total Price: ₹ 57,708.49
Antigen
TPH1
Classification
Polyclonal
Formulation
0.01M HEPES with 0.15M NaCl, 100μg/mL BSA, 50% glycerol and no preservative; pH 7.5
Gene Accession No.
P09810, P17290, P17752
Gene Symbols
TPH1
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Rabbit, Rat
Product Type
Antibody
Isotype
IgG
Applications
Immunohistochemistry, Immunohistochemistry (Frozen), Western Blot
Conjugate
Unconjugated
Gene
TPH1
Gene Alias
indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; Tph1; TPH2; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
Host Species
Sheep
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
100009100, 100101558, 24848, 7166
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- This antibody is predicted to react with wide variety of mammals based on 100% sequence homology, however it does not recognize TPH in rabbit tissues
- This antibody is specific for the ∽55 kDa TPH protein in Western blots of human dorsal Raphe nucleus
- Immunolabeling has been demonstrated in rat brain extracts
- Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001)
- In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland
- Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis
- Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland
- The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996)
- Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).