SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI700048-Each-of-1 | In Stock | ₹ 57,138.00 |
PI700048 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,138.00
GST (18%): ₹ 10,284.84
Total Price: ₹ 67,422.84
Antigen
SMAD2
Classification
Recombinant Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide
Gene Accession No.
Q15796
Gene Symbols
SMAD2
Immunogen
A peptide corresponding to amino acids 81-107 of Q15796.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
ChIP Assay, ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Clone
31H15L4
Conjugate
Unconjugated
Gene
SMAD2
Gene Alias
7120426M23Rik; EGK_09664; hMAD-2; hSMAD2; JV18; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Madh2; Madr2; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic-like protein 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2; Smad-2; Smad2Deltaexon3
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
4087
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- This antibody is predicted to react with bovine, carp, chicken, goldfish, mouse, opossum, orangutan, rat, Xenopus and zebrafish based on sequence homology
- Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation
- SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling
- Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin
- In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors
- The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4
- The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors
- SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin
- Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.