Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI700062-Each-of-1 | In Stock | ₹ 56,203.50 |
PI700062 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,203.50
GST (18%): ₹ 10,116.63
Total Price: ₹ 66,320.13
Antigen
Vinculin
Classification
Recombinant Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide
Gene Accession No.
P18206, Q64727
Gene Symbols
VCL
Immunogen
A recombinant protein corresponding to amino acids 408-591 of P18206.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Clone
42H89L44
Conjugate
Unconjugated
Gene
VCL
Gene Alias
9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
22330, 7414
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain
- This antibody reacts with Human Vinculin
- Based on sequence similarity, reactivity to chimpanzee, Rhesus monkey, swine, equine, mouse, rat, bovine, and chicken is expected
- Immunogen sequence IRGALAEARKIAELCDDPKERDDILRSLGEI SALTSKLADLRRQGKGDSPEARALAKQV ATALQNLQTKTNRAVANSRPAKAAVHLE GKIEQAQRWIDNPTVDDRGVGQAAIRGL VAEGHRLANVMMGPYRQDLLAKCDRVD QLTAQLADLAARGEGESPQARALASQLQ DSLKDLKARMQEAM
- Test material for western blotting/immunofluorescence: HeLa cells
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions
- It functions as one of several interacting proteins involved in anchoring F-actin to the membrane
- It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques
- In the assembly of adhesion plaques, the beta subunit of integrin binds to talin
- Talin binds to vinculin that interacts with alpha-actinin and possibly with itself
- Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system
- Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.