IkB alpha Recombinant Rabbit Monoclonal Antibody (6H7L22), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI701098-Each-of-1 | In Stock | ₹ 44,544.50 |
PI701098 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,544.50
GST (18%): ₹ 8,018.01
Total Price: ₹ 52,562.51
Antigen
IkB alpha
Classification
Recombinant Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide
Gene Accession No.
P25963
Gene Symbols
NFKBIA
Immunogen
Recombinant protein corresponding to amino acids 1-110 of human NF-kappa-B inhibitor alpha
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Immunohistochemistry (Paraffin), Western Blot
Clone
6H7L22
Conjugate
Unconjugated
Gene
NFKBIA
Gene Alias
AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
4792
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
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Description
- Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity
- The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol
- The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus
- A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha
- These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses
- In humans, the gene is located on the q arm of chromosome 14
- Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB
- IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36
- IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines
- Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1
- The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.