Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA514512-Each-of-1 | In Stock | ₹ 54,023.00 |
PIMA514512 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,023.00
GST (18%): ₹ 9,724.14
Total Price: ₹ 63,747.14
Antigen
Cyclin D1
Classification
Recombinant Monoclonal
Concentration
0.045 mg/mL
Formulation
PBS with 1% BSA and 0.1% sodium azide; pH 7.2
Gene Accession No.
P24385, P25322, P39948
Gene Symbols
CCND1
Immunogen
A synthetic peptide from C-terminus of human cyclin D1
Quantity
500 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone
SP4
Conjugate
Unconjugated
Gene
CCND1
Gene Alias
AI327039; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cD1; cDNA; CycD1; cyclin D1; Cyl-1; D11S287E; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD1; PRAD-1; PRAD1 oncogene; U21B31
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
12443, 58919, 595
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- MA5-14512 targets Cyclin D1/Bcl-1 in IHC (P), ICC/IF and WB applications and shows reactivity with Human and Mouse samples
- The MA5-14512 immunogen is a synthetic peptide from C-terminus of human cyclin D1
- Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein
- Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL)
- In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation
- In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11
- Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation
- D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1
- Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation
- Cyclin D1 destabilization participates in G1/S phase arrest
- The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle
- Cyclins function as regulators of CDK kinases
- Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event
- Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition
- The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb
- Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis
- Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.