GFP Monoclonal Antibody (GF28R), Invitrogen™

Description

• MA5-15256 recognizes native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP
• MA5-15256 has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications
• MA5-15256 may be stored at 4oC short term
• Add 0.05% sodium azide if desired
• For long term storage, store at -20oC, avoiding freeze/thaw cycles
• The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal
• GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms
• As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted
• GFP technology has revealed considerable new insights in the physiological activities of living cells
• GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment
• The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors
• GFP fluorescence is stable, species independent and is suitable for a variety of applications
• GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization
• Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.