PIMA516982
CD33 Monoclonal Antibody (WM53), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA516982-Each-of-1 | In Stock | ₹ 29,414.50 |
PIMA516982 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,414.50
GST (18%): ₹ 5,294.61
Total Price: ₹ 34,709.11
Antigen
CD33
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 0.09% sodium azide
Gene Accession No.
P20138
Gene Symbols
CD33
Immunogen
Human AML cells
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
Applications
Flow Cytometry, Immunoprecipitation, Western Blot
Clone
WM53
Conjugate
Unconjugated
Gene
CD33
Gene Alias
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3
Host Species
Mouse
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
945
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Form
Liquid
Related Products
Description
- Overnight incubation is recommended
- The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing
- We recommend the use of acetone fixation for frozen sections
- A suggested positive control for immunohistochemical applications is lymphatic tissue
- For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
- Overnight incubation with primary antibody is recommended
- CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family
- It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface
- CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia
- Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells
- CD33 is also expressed on, and is a useful marker for, peripheral monocytes
- CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias
- Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.