PIPA5143657
Blasticidin S Deaminase Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5143657-Each-of-1 | In Stock | ₹ 47,570.50 |
PIPA5143657 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,570.50
GST (18%): ₹ 8,562.69
Total Price: ₹ 56,133.19
Antigen
Blasticidin S Deaminase
Classification
Polyclonal
Conjugate
Unconjugated
Gene
BAD
Gene Alias
blasticidin; BSD
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus Terreus.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Fungi
Form
Liquid
Applications
Immunocytochemistry, Western Blot
Concentration
∽0.25 mg/mL
Formulation
PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene Accession No.
Q61337
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
12015
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Isotype
IgG
Related Products
Description
- The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells
- Retroviral vectors are commonly used for gene expression in vitro and in vivo
- The structure of these vectors usually consists of one gene of interest and a selectable marker gene
- Fast selection without damaging cells is a critical step for significant gene expression
- Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin
- A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin
- This selectable marker allows selection of stable mammalian cell lines in as little as one week.