TID1 Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA529225-Each-of-1 | In Stock | ₹ 46,502.50 |
PIPA529225 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 46,502.50
GST (18%): ₹ 8,370.45
Total Price: ₹ 54,872.95
Antigen
TID1
Classification
Polyclonal
Conjugate
Unconjugated
Gene
DNAJA3
Gene Alias
1200003J13Rik; 1810053A11Rik; C81173; DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ heat shock protein family (Hsp40) member A3; dnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Dnaja3; HCA57; Hepatocellular carcinoma-associated antigen 57; hTID-1; mTid-1; Tid1; Tid-1; Tid1l; Tumorous imaginal discs protein Tid56 homolog
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
360481, 9093
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration
1 mg/mL
Formulation
0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
Gene Accession No.
Q96EY1
Gene Symbols
DNAJA3
Immunogen
Recombinant fragment corresponding to a region within amino acids 172 and 438 of Human TID1
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (80%), Bovine (95%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
- TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L
- These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70
- They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity
- Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a
- A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis
- Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected
- It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities
- Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.