Invitrogen™ GeneBLAzer™ ADORA2A CHO-K1 DA Assay Kit

Description

• This cell line is available as dividing cells by requesting a quote for K1245 from dividingcells@invitrogen.com GeneBLAzer™ ADORA2A CHO-K1 DA (Division Arrested) cells and ADORA2A-CRE- bla CHO-K1 cells contain the human Adenosine A2a (ADORA2A) receptor stably integrated into the CellSensor™ CRE- bla CHO-K1 cell line
• CellSensor™ CRE- bla CHO-K1 cells (Cat
• no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE)
• Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
• DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
• Both ADORA2A CHO-K1 DA cells and ADORA2A-CRE- bla CHO-K1 cells are functionally validated for Z’-factor and EC 50 concentrations of 5’-adenosine (NECA); (Figure 1)
• In addition, ADORA2A-CRE- bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
• Additional testing data using alternate stimuli are also available
• The Adenosine A2a Receptor (ADORA2A) has been shown to have many roles throughout the body
• ADORA2A is involved in pain and inflammation regulation, inhibition of platelet aggregation, blood pressure regulation and contributes to ischemic brain damage
• Selective antagonists of the ADORA2A pathway are being tested as adjuvants to dopaminergic drugs to treat Parkinson’s disease and schizophrenia
• Storage: GeneBLAzer™ ADORA2A CHO-K1 DA Cells and ADORA2A-CRE- bla CHO-K1 Cells are shipped on dry ice
• Store in liquid nitrogen immediately upon receipt or thaw for immediate use
• Contents: GeneBLAzer™ ADORA2A CHO-K1 DA Assay Kit Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate
• (Other materials are required separately; please refer to the protocol)
• Includes: ADORA2A CHO-K1 DA cells LiveBLAzer™-FRET B/G Loading Kit, 70μg Solution D, 1ml 2 protocols Certificate of Analysis GeneBLAzer™ ADORA2A CHO-K1 DA cells Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
• Includes: ADORA2A CHO-K1 DA cells 1 protocol Certificate of Analysis GeneBLAzer™ ADORA2A-CRE- bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
• Includes: ADORA2A-CRE- bla CHO-K1 cells 1 protocol Certificate of Analysis