K1393
Invitrogen™ GeneBLAzer™ ER Alpha DA Assay Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1393-Each-of-1 | In Stock | ₹ 1,29,584.00 |
K1393 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,29,584.00
GST (18%): ₹ 23,325.12
Total Price: ₹ 1,52,909.12
Alternate Agonists
Betamethasone, Cortisol, Budesonide
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER alpha GripTite™ DA cells (K1391A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez)
2099
Primary Agonist
Dexamethasone
Quantity
1 plate
Target Entry
ER alpha, ESR1, ERA, NR3A1
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
Milfepristone, Cortisone, Progesterone
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
ERα
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ER alpha DA(Division Arrested) cells and ER alpha-UAS-bla GripTite™ cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ cell line
- GeneBLAzer™UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both ER alpha DA cells and ER alpha-UAS-bla GripTite™cells are functionally validated for Z' and EC 50 concentrations of 17-beta Estradiol
- In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.