11-102-1816
TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-102-1816-Each-of-1 | In Stock | ₹ 2,23,746.00 |
11-102-1816 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,23,746.00
GST (18%): ₹ 40,274.28
Total Price: ₹ 2,64,020.28
Diameter (Metric)
7.5 mm
Length (Metric)
7.5 cm
Max. Pressure
10 bar
pH
2 to 12
Solvents
≤ 20% Organic Solvent
Stationary Phase
Iminodiacetic Acid
Matrix
Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
Flow Rate
1.2 mL/min
Ligand Type
Resin Ligand Density: 20 μmol/mL
Particle Size
10 μm
Pore Size
1000 Å
Temperature
≤45°C
Product Line
TSKgel
Technique
Affinity
Related Products
Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+ Column is loaded with divalent metal ions by chelation Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH Retained compounds can be eluted with buffer containing imidazole or glycine A decreasing pH gradient can be used for elution purposes as well For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides