11-102-1817
TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-102-1817-Each-of-1 | In Stock | ₹ 8,99,345.00 |
11-102-1817 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,99,345.00
GST (18%): ₹ 1,61,882.10
Total Price: ₹ 10,61,227.10
Column Format
72% (v/v) Slurry in 20% (v/v) Ethanol
Flow Rate
1 mL/min
Ligand Type
Resin Ligand Density: 20 μmol/mL
Particle Size
13 μm
Solvents
≤ 20% Organic Solvent
Stationary Phase
Iminodiacetic Acid
Matrix
Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
Technique
Affinity
Diameter (Metric)
21.5 mm
Length (Metric)
15 cm
Max. Pressure
15 bar
pH
2 to 12
Temperature
≤45°C
Product Line
TSKgel
Material
Stainless Steel
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Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+ Column is loaded with divalent metal ions by chelation Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH Retained compounds can be eluted with buffer containing imidazole or glycine A decreasing pH gradient can be used for elution purposes as well For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides