F170S
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F170S-Each-of-1 | In Stock | ₹ 26,744.50 |
F170S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,744.50
GST (18%): ₹ 4,814.01
Total Price: ₹ 31,558.51
Content And Storage
• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL• Dilution Buffer, 5 mL• DNARelease Additive, 3 x 100 μL• 25 μM Control Primer Mix, 40 μL• Nuclease-free water, 2 x 1.25 mL• 50 μg O'GeneRuler Express DNA LadderStore at -20°C.
PCR Method
Direct PCR
Reaction Speed
Fast
Product Type
Direct PCR Master Mix
For Use With (Application)
Genotyping, Hot-start PCR
Hot Start
Built-In Hot Start
Overhang
Blunt
Sample Type
Tissue
GC-Rich PCR Performance
Medium
Polymerase
Phire Hot Start II DNA Polymerase
Includes Label or Dye
Yes
Shipping Condition
Wet or Dry Ice
Concentration
2X
No. of Reactions
250 Reactions
Reaction Format
SuperMix or Master Mix
Starting Material
DNA
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Description
- Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects
- The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle)
- The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues
- Features Direct PCR reduces time-consuming and expensive DNA purification steps Available Dilution & Storage protocol allows multiple PCR reactions from a single sample Specially engineered Phire DNA polymerase for extremely short PCR protocol times Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel Applications Genotyping Transgene detection Knockout analysis Sequencing Notes The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.