F170SPM
Thermo Scientific™ Phire Tissue Direct PCR Master Mix PROMO
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F170SPM-Each-of-1 | In Stock | ₹ 14,786.46 |
F170SPM - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,786.46
GST (18%): ₹ 2,661.563
Total Price: ₹ 17,448.023
Content And Storage
Contains: 2 x 1.25 mL Phire Tissue Direct PCR Master Mix (2X), 5 mL Dilution Buffer, 3 x 100 μL DNARelease Additive, 40 μL Control Primer Mix (25 μM each), 2 x 1.25 mL Water, nuclease- free, 50 μg O'GeneRuler Express DNA Ladder (sufficient for 100 applications), Store at -20°C.
PCR Method
Direct PCR
Reaction Speed
Fast
Product Line
Phire
Quantity
250 x 20 μL Reactions
Hot Start
Built-In Hot Start
Overhang
Blunt
Sample Type
Tissue
GC-Rich PCR Performance
Medium
Polymerase
Phire Hot Start II DNA polymerase
Includes Label or Dye
Yes
Product Type
Direct PCR Master Mix
For Use With (Application)
Genotyping
No. of Reactions
250 x 20 mL Reactions
Reaction Format
SuperMix or Master Mix
Starting Material
DNA
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Description
- Description The Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects
- The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle)
- The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues
- Highlights Direct PCR – sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps Dilution and Storage protocol available – allows tens of PCR reactions from one tiny sample and allows re-testing Specially engineered Phire DNA polymerase – extremely short PCR protocol times Master Mix format with premixed gel loading dye – minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel Applications Genotyping Transgene detection Knockout analysis Sequencing Includes 2X Phire Tissue Direct PCR Master Mix Reaction voume with dilution and storage protocol: 20 μL Reaction voume with direct protocol: 50 μL Dilution Buffer DNARelease Additive Universal control primer mix (25 μM each) Water, nuclease- free O'GeneRuler Express DNA Ladder