70-726-3
MilliporeSigma™ Novagen™ Tuner™ Competent Cell Set
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 70-726-3-Each-of-1 | In Stock | ₹ 34,766.96 |
70-726-3 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,766.96
GST (18%): ₹ 6,258.053
Total Price: ₹ 41,025.013
Content And Storage
≤-70°C
Quantity
1 Set
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Description
- The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction
- By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors
- Lower-level expression may enhance the solubility and activity of difficult target proteins
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.