70-911-3
MilliporeSigma™ Novagen™ Origami™ B Competent Cell Set
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 70-911-3-Each-of-1 | In Stock | ₹ 34,766.96 |
70-911-3 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 34,766.96
GST (18%): ₹ 6,258.053
Total Price: ₹ 41,025.013
Content And Storage
≤-70°C
Quantity
1 Set
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Description
- Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™ and Origami hosts in one strain background
- The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.