CD83 Monoclonal Antibody (HB15e), eBioscience™, Invitrogen™

Description

• Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein
• CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen
• While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells
• Applications Reported: The HB15e antibody has been reported for use in flow cytometric analysis
• Applications Tested: The HB15e antibody has been tested by flow cytometric analysis of human peripheral blood-derived dendritic cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells
• Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA
• In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres
• CD83 is also expressed by Langerhan's cells in the skin
• The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail
• CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein
• However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage
• CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages
• They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules
• Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.