NKG2A/C/E Monoclonal Antibody (20d5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E
• NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages
• NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1
• When co-staining with NKG2A^B6 clone 16a11, it is important to stain for NKG2A-B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently
• Applications Reported: This 20d5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 20d5 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation
• They can also regulate specific humoral and cell-mediated immunity
• The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells
• This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain
• This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells
• The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12
• Four alternatively spliced transcript variants encoding two distinct isoforms have been observed.