MUC-1 Ab-5 Hamster Monoclonal Antibody, Epredia™

Description

• Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells
• It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases
• In normal mammary gland it is expressed in apical surface of glandular epithelium
• In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost
• Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other
• Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1
• Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation
• Host Species: Hamster Clone: MH1 (CT2) Isotype: IgG Species Reactivity: Human and Mouse
• Others not tested
• Epitope: aa 239-255 Immunogen: A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) from the cytoplasmic tail of MUC-1 Molecular Weight: 14-28kDa Positive Control: MCF-7 cells; Breast CA Cellular Localization: Cytoplasmic and membrane Recommended for: Immunohistochemistry (Formalin/paraffin) Western Blotting Immunofluorescence Immunoprecipitation Flow Cytometry Note: USA: RUO; Int'l: RUO