Sall1 Monoclonal Antibody (NRNSTNX), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1
• Applications Reported: This NRNSTNX antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This NRNSTNX antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse brain cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol
• Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor 450 is an alternative to Pacific Blue
• eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex
• It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity
• Gene expression profiling has identified Sall1 as a microglial signature gene
• Microglia are the resident macrophages of the central nervous system (CNS)
• Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes
• Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells
• Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles
• Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome
• Heterozygous mutations of human SALL1 leading to Townes–Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al
• 1998)
• Two transcript variants encoding different isoforms have been found for this gene.