4840480
CD8a Rat anti-Mouse, BB515, Clone: 53-6.7, BD
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD8a
Concentration
0.2mg/mL
Classification
Monoclonal
Host Species
Rat
Target Species
Mouse
Isotype
IgG2a κ
Purification Method
Affinity Purified
Description
Cd8a; CD8 alpha chain; Ly-2; Lyt2; Lyt-2; Ly-35; Ly-B
Clone
53-6.7
Applications
Flow Cytometry
Conjugate
Brilliant Blue 515 (BB515)
Regulatory Status
RUO
Immunogen
Mouse Spleen Cells or Thymocyte Membranes
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Description
- The 53-6.7 antibody monoclonal antibody specifically binds to the 38 kDa α and 34 kDa α chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested
- The CD8 α and α chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes
- A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the αα chains arise from alternatively spliced messengers of a single CD8a gene
- The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b
- The truncated α chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation
- In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes
- The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis .