50-108-64
NOTCH2 Monoclonal Antibody (16F11), PE, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-108-64-Each-of-1 | In Stock | ₹ 31,595.00 |
50-108-64 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,595.00
GST (18%): ₹ 5,687.10
Total Price: ₹ 37,282.10
Antigen
NOTCH2
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
O35516, Q04721
Gene Symbols
NOTCH2
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
18129, 4853
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
16F11
Conjugate
PE
Gene
NOTCH2
Gene Alias
AGS2; AI853703; HJCYS; hN2; Motch B; N2; N2ECD; N2ICD; Neurogenic locus notch homolog protein 2; notch 2; Notch 2 extracellular truncation; Notch 2 intracellular domain; notch gene homolog 2; Notch homolog 2; notch receptor 2; Notch2
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: This 16F11 monoclonal antibody reacts with the extracellular domain of human and mouse Notch2, one of four members of the Notch family of receptors
- Notch receptors are 300-kDa single-pass transmembrane proteins
- While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling
- Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
- The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription
- Notch2 is expressed on thymocytes (i.e., DN1 and DN2 subsets), activated peripheral T cells, and bone marrow
- Moreover, expression of Notch2 has been reported to be altered in various cancers and lymphomas
- In addition to its role in stem cell hematopoiesis, Notch2 plays a pivotal role in marginal zone B cell development
- Notch2 also interacts with phosphorylated CREB1 to induce granzyme B transcription in cytotoxic T cells
- Applications Reported: This 16F11 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 16F11 antibody has been tested by flow cytometric analysis of 24-hour anti-CD3-stimulated mouse splenocytes
- Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved Notch signaling pathway protein
- NOTCH2 is a type 1 transmembrane protein with an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types
- NOTCH2 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination
- Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2)
- Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations
- There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.