STRO-1 Monoclonal Antibody (STRO-1), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody STRO-1 recognizes STRO-1, a bone marrow (immature) mesenchymal cell surface protein
• Pluripotent stromal cells can give rise to several lineages: osteoblasts and chondrocytes, which form bone, adipocytes or hematopoietic cells
• STRO-1+ cells when co-transplanted with hematopoietic stem cells have been shown to reduce the occurrence of graft versus host disease
• In addition, in vitro cultures of Stro-1+ cells along with CD34+ cells give rise to CFUs (colony forming units) composed of hematopoietic precursors, establishing a supporting role of these mesenchymal cells in blood cell development
• The STRO-1 antibody has been reported to recognize both bovine and rat Stro-1 protein
• Applications Reported: This STRO-1 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, and immunohistochemical staining of frozen tissue sections
• Applications Tested: This STRO-1 antibody has been tested by immunocytochemistry on methanol-fixed MG-63 cells and can be used at less than or equal to 20 μg/mL
• The STRO-1 antibody has also been tested by flow cytometry on MG-63 cells and can be used at less than or equal to 0.5 μg/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow
• STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix)
• From bone marrow cells, the frequency of fibroblast colony forming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population
• When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements
• A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes
• STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.