Thrombospondin-1 Monoclonal Antibody (A6.1), eBioscience™, Invitrogen™

Description

• Description: The A6.1 monoclonal antibody recognizes human, mouse, and rat thrombospondin-1, a member of the thrombospondin family of extracellular calcium-binding proteins
• Thrombospondin-1 is a secreted glycoprotein that was first discovered in activated platelets but is also expressed by endothelial cells, adipocytes, fibroblasts, macrophages, monocytes, and smooth muscle cells
• Thrombospondin-1 functions by binding members of the extracellular matrix such as fibronectin, integrins, and heparin, and its signaling is mediated through activation of the TGF beta signaling pathway
• Thrombospondin-1 has been shown to be involved in angiogenesis, inflammation, osteogenesis, cell proliferation, and synaptogenesis during neural development
• Applications Reported: This A6.1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry
• Applications Tested: This A6.1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 μg/mL
• This A6.1 antibody has been tested by immunocytochemistry of formaldehyde or methanol fixed and permeabilized human cells and can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Thrombospondin is a protein from platelet a-granules
• It is made up of three identical subunits bound by interchain disulfides
• It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.