50-112-3655

Vinculin Monoclonal Antibody (7F9), eFluor™ 570, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-3655-Each-of-1 In Stock ₹ 40,050.00

50-112-3655 - Each of 1

₹ 40,050.00

In Stock

Quantity

1

Base Price: ₹ 40,050.00

GST (18%): ₹ 7,209.00

Total Price: ₹ 47,259.00

Antigen

Vinculin

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P18206, P85972, Q64727

Gene Symbols

VCL

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

22330, 305679, 7414

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Clone

7F9

Conjugate

eFluor 570

Gene

VCL

Gene Alias

9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human, Mouse, Rat

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin
  • Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions
  • Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression
  • Vinculin binds to talin, paxillin, and alpha-actinin
  • Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis
  • Applications Reported: This 7F9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry
  • Applications Tested: This 7F9 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70)
  • A standard Alexa Fluor™ 555 or TRITC filter is acceptable
  • Excitation: 555 nm; Emission: 570 nm Vinculin Monoclonal antibody specifically detects Vinculin in Human, Mouse, Rat samples
  • It is validated for Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).