Nestin Monoclonal Antibody (10C2), eFluor™ 570, eBioscience™, Invitrogen™

Description

• The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614)
• Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system
• Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential
• Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP
• Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors
• No cross-reactivity to rodent nestins has been observed using the 10C2 antibody
• Applications Reported: This 10C2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry
• Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized human cells and can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filter Recommendation: When using this eFluor 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70)
• A standard Alexa Fluor 555 or TRITC filter is acceptable
• Excitation: 555 nm; Emission: 570 nm Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
• Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
• Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
• Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
• Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.