IgD Monoclonal Antibody (10.4.22), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells
• This antibody has been reported to activate B cells via engagement of the IgD receptor
• The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM
• Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c
• Applications Reported: This 10.4.22 antibody has been reported for use in functional assays
• Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.