50-139-53

Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-139-53-Each-of-1 In Stock ₹ 15,931.00

50-139-53 - Each of 1

₹ 15,931.00

In Stock

Quantity

1

Base Price: ₹ 15,931.00

GST (18%): ₹ 2,867.58

Total Price: ₹ 18,798.58

Antigen

Gata-4

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P43694, Q08369

Gene Symbols

GATA4

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

14463, 2626

Content And Storage

4° C

Form

Liquid

Applications

Immunohistochemistry (Paraffin), Western Blot

Clone

eBioEvan

Conjugate

Unconjugated

Gene

GATA4

Gene Alias

ASD2; DNA-binding protein GATA-GT2; GAT4; GATA binding protein 4; GATA4; Gata-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; TACHD; TOF; Transcription factor GATA-4; VSD1

Host Species

Rat

Quantity

25 μg

Primary or Secondary

Primary

Target Species

Human, Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4
  • In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights
  • The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4
  • Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers
  • The mouse Gata-4 gene encodes a protein of 48kDa
  • Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival
  • Gata-4 is expressed during fetal development, as well as in the adult mouse heart
  • It also appears to modulate gene expression in the gut, liver, and gonads
  • Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry
  • Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates
  • As a starting dilution, it is recommended to use this purified antibody at 2μg/mL for western blot analysis
  • The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL
  • GATA4 is a member of the GATA family of zinc-finger transcription factors
  • Members of this family recognize the GATA motif which is present in the promoters of many genes
  • This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function
  • Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.