Gata-4 Monoclonal Antibody (eBioEvan), eBioscience™, Invitrogen™

Description

• Description: The eBioEvan monoclonal antibody reacts with mouse and human Gata-4
• In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights
• The eBioEvan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4
• Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers
• The mouse Gata-4 gene encodes a protein of 48kDa
• Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival
• Gata-4 is expressed during fetal development, as well as in the adult mouse heart
• It also appears to modulate gene expression in the gut, liver, and gonads
• Applications Reported: This eBioEvan antibody has been reported for use in immunoblotting (WB) and immunohistochemistry
• Applications Tested: This eBioEvan antibody has been tested by western blot analysis of mouse heart lysates
• As a starting dilution, it is recommended to use this purified antibody at 2μg/mL for western blot analysis
• The eBioEvan antibody has been tested on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL
• GATA4 is a member of the GATA family of zinc-finger transcription factors
• Members of this family recognize the GATA motif which is present in the promoters of many genes
• This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function
• Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.