50-155-77
FR4 Monoclonal Antibody (eBio12A5), PE-Cyanine7, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-155-77-Each-of-1 | In Stock | ₹ 18,957.00 |
50-155-77 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 18,957.00
GST (18%): ₹ 3,412.26
Total Price: ₹ 22,369.26
Antigen
FR4
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
Q9EQF4
Gene Symbols
Izumo1r
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
64931
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
eBio12A5
Conjugate
PE-Cyanine7
Gene
Izumo1r
Gene Alias
0910001L11Rik; C86255; Folate receptor 4; folate receptor 4 (delta); folate receptor 4 (delta) homolog; folate receptor 4, delta (putative); folate receptor delta; Folate-binding protein 3; Folbp3; Folr4; FR-delta; IZUMO1 receptor protein JUNO; IZUMO1 receptor, JUNO; Izumo1r; Juno; probable folate receptor delta; sperm-egg fusion protein Juno
Host Species
Rat
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2b κ
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Description
- Description: The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR delta and FBP3 (folate binding protein3)
- FR4 is a heavily glycosylated, 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
- Natural Treg have high levels of FR4 and together with CD25 can be used to distinguish natural Treg, effector T cells, memory-like T cells and naive T cells
- FR4 high CD25 high cells are natural Treg with high levels of Foxp3
- FR4 high CD25 low cells are identified as central memory T cells, which upon stimulation, can proliferate and produce large amounts of IL-2
- In contrast, FR4 low CD25 high cells secrete proinflammatory cytokines such as IFN gamma and IL-17 and have been characterized as effector memory T cells
- Based on co-staining studies, the epitopes recognized by eBioTH6 and eBio12A5 are different, thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5
- Applications Reported: This eBio12A5 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBio12A5 antibody has been tested by flow cytyometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
- Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naive T cells
- In vivo studies with eBioTH6 show a decrease in the number of CD4+CD25+ cells and an enhanced tumor response leading to the elimination of advanced stages of tumors.