CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-160-17-Each-of-1 | In Stock | ₹ 12,638.00 |
50-160-17 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 12,638.00
GST (18%): ₹ 2,274.84
Total Price: ₹ 14,912.84
Antigen
CD154 (CD40 Ligand)
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P27548
Gene Symbols
CD40LG
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
21947
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
MR1
Conjugate
PerCP-eFluor 710
Gene
CD40LG
Gene Alias
CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
Host Species
Armenian Hamster
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG
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Description
- Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L)
- CD154 is expressed transiently by activated T cells
- Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction
- CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle
- For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1
- Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
- CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells
- The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines
- CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes
- It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.