CD154 (CD40 Ligand) Monoclonal Antibody (24-31), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD154 (CD40 Ligand)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
Gene Symbols
CD40LG
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
CD40LG
Clone
24-31
Applications
Flow Cytometry
Conjugate
APC
Host Species
Mouse
Target Species
Cynomolgus Monkey, Human
Gene Accession No.
P29965
Gene ID (Entrez)
102138630, 959
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L)
- CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells
- Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction
- CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry
- 24-31 cross-reacts with the cynomolgus monkey CD154
- Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of activated normal human peripheral blood cells
- This can be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells
- The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines
- CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes
- It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.