50-199-1877

Phospho-ECT2 (Thr790)Rabbit anti-Canine, Chicken, Chimpanzee, Human, Mouse, Rat, Zebrafish, Polyclonal, Rockland™

Manufacturer: Rockland Immunochemicals

Select a Size

Pack Size SKU Availability Price
Each of 1 50-199-1877-Each-of-1 In Stock ₹ 52,930.08

50-199-1877 - Each of 1

₹ 52,930.08

In Stock

Quantity

1

Base Price: ₹ 52,930.08

GST (18%): ₹ 9,527.414

Total Price: ₹ 62,457.494

Antigen

Phospho-ECT2 (Thr790)

Classification

Polyclonal

Conjugate

Unconjugated

Gene

ECT2

Gene Alias

AI528536; ARHGEF31; BOS_1061; Ect2; ect2 oncogene; ect2 protein; epithelial cell transforming 2; epithelial cell transforming sequence 2 oncogene; epithelial cell transforming sequence 2 oncogene protein; Epithelial cell-transforming sequence 2 oncogene; FLJ10461; LOW QUALITY PROTEIN: protein ECT2; MGC138291; mKIAA4037; Protein ECT2

Host Species

Rabbit

Regulatory Status

RUO

Gene ID (Entrez)

13605, 1894, 361921, 424983, 445406, 460853, 488172

Content And Storage

-20°C, Avoid Freeze/Thaw Cycles

Form

Liquid

Applications

ELISA, Immunoprecipitation, Western Blot

Concentration

1 mg/mL

Formulation

0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2

Gene Accession No.

Q07139, Q9H8V3

Gene Symbols

ECT2

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Canine, Chicken, Chimpanzee, Human, Mouse, Rat, Zebrafish

Product Type

Antibody

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Description

  • Store vial at -20°C prior to opening
  • Aliquot contents and freeze at -20°C or below for extended storage
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue
  • Reactivity occurs against human ECT2 protein at the pT790 residue
  • By ELISA the antibody is specific for the phosphorylated form of the protein
  • Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2
  • Additional experiments are underway to clarify antibody specificity
  • A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence
  • Reactivity against homologues from other sources is not known
  • The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators
  • The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases
  • In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver
  • Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.