Perforin Monoclonal Antibody (dG9 (delta G9)), APC, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 50-245-716-Each | In Stock | ₹ 32,364.85 |
50-245-716 - Each
In Stock
Quantity
1
Base Price: ₹ 32,364.85
GST (18%): ₹ 5,825.673
Total Price: ₹ 38,190.523
Antigen
Perforin
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P14222
Gene Symbols
PRF1
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
396595, 5551
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
dG9 (delta G9)
Conjugate
APC
Gene
PRF1
Gene Alias
Cyta; Cytolysin; FLH2; HPLH2; lymphocyte pore forming protein; lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1; perforin 1 (pore forming protein); Perforin1; perforin-1; Pfn; PFN1; Pfp; PGFL; PIGF; PIGF-2; PLGF; pore forming protein; pore-forming protein; PRF1; Prf-1; PRF1 (pore forming protein 1); RATCYTA; RP11-710A11.3
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human, Porcine
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp)
- Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK)
- Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections
- Applications Reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.06 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Perforin is one of the major cytolytic proteins of cytolytic granules
- Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules
- Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes
- Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD
- Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated
- Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested
- Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis
- Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood
- Alternative splicing results in multiple transcript variants of perforin.