Podocalyxin Monoclonal Antibody (B34D1.3), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG)
• Podocalxyin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan
• It was first identified on kidney podocytes, and then found to be upregulated in a variety of human cancers such as breast and prostate cancer
• Like other stem cell-specific markers, the epitope recognized by the B34D1.3 antibody is lost upon cell differentiation
• The B34D1.3 antibody recognizes the ectodomain of podocalyxin, which is a different epitope from the Tra-1-60 and Tra-1-81 monoclonal antibodies
• While Tra1-60 has been found to react with a sialidase-sensitive epitope, the epitope recognized by Tra1-81 has not been fully defined
• Applications Reported: This B34D1.3 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This B34D1.3 antibody has been pre-titrated and tested by flow cytometric analysis of the NTERA cell line
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a member of the sialomucin protein family
• The encoded protein was originally identified as an important component of glomerular podocytes
• Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane
• Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.