5349257
MilliporeSigma™ anti-CD4 Red Fluor 710, Clone: RM4-5,
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD4
Applications
Flow Cytometry
Conjugate
Red Fluor 710
Host Species
Rat
Research Discipline
Inflammation, Immunology
Formulation
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols
Cd4
Primary or Secondary
Primary
Content And Storage
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone
RM4-5
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Accession No.
P06332
Immunogen
Proprietary immunogen
Purification Method
Purified by size-exclusion chromatography
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Description
- Specifically detects CD4 Clone: RM4-5 in Mouse samples, and it is validated for Flow Cytometry The RM4-5 antibody reacts with mouse CD4, a 55kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells
- The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation
- CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression
- If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will 'compete' for binding, i.e
- RM4-5 antibody is able to block GK1.5 antibody binding to cells
- In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.