5540644

Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Golgi Glycoprotein 1/GLG1

Concentration

0.2mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human, Mouse (Negative), Rat (Negative)

Gene Accession No.

Q92896, Q92896

Gene ID (Entrez)

2734

Immunogen

Golgi fraction from human liver cells

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

134 kDa

Clone

GLG1/970

Dilution

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160

Gene Symbols

GLG1

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three fun

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Description

  • Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence Golgi Glycoprotein 1/GLG1 Monoclonal specifically detects Golgi Glycoprotein 1/GLG1 in Human, Mouse (Negative), Rat (Negative) samples
  • It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.