Golgi Complex Antibody (AE-6), Novus Biologicals™

Golgi Complex

0.2mg/mL

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry

Unconjugated

Mouse

Human, Mouse (Negative), Rat (Negative)

SU-DHL-1 large cell lymphoma cells

Primary

Store at 4C.

AE-6

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml

Monoclonal

Purified

RUO

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

IgG1 κ

Protein A or G purified

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside