HSP27 Antibody (HSPB1/774), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
HSP27
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols
HSPB1
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Clone
HSPB1/774
Dilution
Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Chicken, Primate, Sheep
Gene Accession No.
P04792
Gene ID (Entrez)
3315
Immunogen
Recombinant human HSPB1 protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
27 kDa
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Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence HSP27 Monoclonal specifically detects HSP27 in Human, Mouse, Rat, Chicken, Chimpanzee, Sheep samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.