5541201

EpCAM/TROP1 Antibody (EGP40/826) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

EpCAM/TROP1

Concentration

0.2mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, Cancer Stem Cells

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A

Gene Symbols

EPCAM

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Clone

EGP40/826

Dilution

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse (Negative), Rat (Negative)

Gene Accession No.

P16422

Gene ID (Entrez)

4072

Immunogen

A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein.

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

41 kDa

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Description

  • Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence EpCAM/TROP1 Monoclonal specifically detects EpCAM/TROP1 in Human, Mouse (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, SDS-Page, Immunofluorescence.